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KMID : 0613820190290101071
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Journal of Life Science
2019 Volume.29 No. 10 p.1071 ~ p.1079
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The Antioxidant and Anti-aging Effects of Treatment with Schisandra chinensis Seeds Fractions in UVB-irradiated Human HaCaT Cells
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Choi Eun-Young
Sohn Ho-Yong
Lee Jin-Tae
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Abstract
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In this study, we investigated the antioxidant and anti-aging activities of Schisandra chinensis seed fractions by adding them to UVB-irradiated HaCaT cells and analyzing the suppression of matrix metalloproteinases (MMPs). An electron-donating assay, ABTS radical scavenging assay, and hydrogen peroxide scavenging assay showed that the ethyl acetate fraction of S. chinensis seed (SCEAf) has scavenging activities. A collagenase inhibition activity assay showed that SCEAf had inhibitory effects of over 92.3% at 500 μg/ml concentration. An MTT assay was performed to determine the cytotoxicity of SCEAf in HaCaT cells and the results showed that SCEAf increased cellular viability in a concentration-dependent manner. In addition, SCEAf was found to increase the viability of cells irradiatged by UVB 50 mJ/cm2. To examine the anti-aging effects of SCEAf on HaCaT cells irradiated with UVB 50 mJ/cm2, the expression of MMP-1 and -3 was analyzed by western blotting and reverse-transcription polymerase chain reaction. The results showed that MMP-1 and -3 proteins and mRNA levels were downregulated in a concentration-dependent manner in response to SCEAf. These results suggest that SCEAf can prevent aging and alleviate aging symptoms by inhibiting collagenase activity in skin keratinocytes damaged by UVB. Therefore, S. chinensis seeds may have the potential for use as functional ingredients with anti-aging effects in the cosmetic and food industries.
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KEYWORD
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Anti-aging, human HaCaT cells, matrix metallopreinases, Schisandra chinensis seeds
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